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Development of fluorescent biomarkers in two foodborne pathogens to better predict the impact of food processing on its survival and virulence in dairy products

The FluoPath project is funded by the French National Research Agency (ANR) for the period April 2024-March 2028 (AAPG 2023) and accredited by 2 competitive clusters: Vitagora and Valorial. This project brings together 7 partners, including 4 academic research laboratories (UMR PAM, UMR SECALIM, UMR SQPOV and LUBEM), 2 agro-industrial technical institutes (AERIAL and ADRIA) and the dairy interprofession (CNIEL).

The main objective of the FluoPath project is to identify new biomarkers (promoters that induce the expression of genes of interest) coupled to a fluorescent biosensor to gain new insights into the physiological state of two pathogens (Listeria monocytogenes and Bacillus cereus) in dairy environments (milk, diluted model cheese and, if possible, solid model cheese) in relation to the impact of technological perturbations. Ultimately, this knowledge, combined with that available in the scientific literature, will be used to improve predictive models for microbiological risk in dairy products.